UV
/Visible Spectroscopy
Regions
of Electromagnetic spectrum, properties of EMR, atomic and molecular spectra,
Beer - Lambert's law and deviations from Beer's law Principles and theoretical
aspects of UVN/Visible Spectroscopy, electronic transition, effect of
conjugation, concept of chromophore and auxochrome, bathochromic, hypsochromic,
hyperchromic and hypochromic shifts Instrumentation - components of
spectrophotometer, types of spectrophotometers, Solvents and sample handling,
Applications - Qualitative and quantitative analysis - single component
IR
spectroscopy
Principles
and theoretical aspects - Molecular vibrations, Hook’s Law, Intensity and
position of IR bands, Measurement of IR spectrum, finger print region and
characteristic absorption of various functional groups.
Instrumentation
- Spectrophotometer components, Sample preparation and handling Application -
Interpretation of IR spectra of simple organic compounds, quantitative
applications.
i)NMR - A brief introduction to the principle and
instrumentation, chemical shift, spin-spin interaction, shielding and de
shielding.
ii)MS - A brief introduction to the principle and
instrumentation, various methods of ion production and fragmentation
rules.
iii)Fluorescence
spectroscopy - Fundamentals,
radiative and non radiative process, mirror image relation ship, fluorescence
and molecular structure, properties of fluorescence. Instrumentation -
components of spectrofluorimeter and applications
Electrochemical
methods
i)
Amperometric titrations
ii)
Potentiometry - principles and
theoretical aspects - electrodes, measurement of cell potential, end point
evaluation methods, potentiometric titrations, Null point potentiometry and
application.
iii)
Conductometry - principles and
theoretical aspects, conductance, equivalent and molar conductance, effect of
dilution on conductance, conductivity water, cell constant, conductivity cell,
measurement of conductivity, conductimetric titrations and applications. Other
analytical techniques - Principle, Instrumentation and application of following
instrumental methods of analysis nephelometry, turbidometry, flame
photometryand differential thermal analysis
Chromatography: Principle, instrumentation and experimental details
and applications of paper chromatography, TLC, column chromatography, gas
chromatography, HPLC and HPTLC.
Electrophoresis
: Principle, instrumentation,
experimental details and applications of paper and gel electrophoresis .