Genetic
Engineering
Introduction,
History, Development, Application and Scope Genetics, DNA/RNA replication,
Restriction Endonucleases, DNA Ligases, Vectors, Hosts, Cloning strategies, Gene
Expression in Recombinant DNA. Application of recombinant DNA in manufacture of
biological products such as Insulin, Human growth hormones, Interferons and
Interleukins.
Biochemical
Engineering – Fermentation Technology
Introduction, development
and maintenances of industrial micro-organisms, batch and continuous
fermentations, process controls, oxygen supply and demand, single and multiple
bubble aeration, sparger aeration, foam control equipment, scale-up of
Fermentors.
Microbiological Assay of
antibiotics and Vitamin B12 .
Study of culture, media,
production conditions, extraction and purification of the following:
Antibiotics
– Semi synthetic penicillin’s,
streptomycin and erythromycin as per IP.
Hormones - Insulin Production
Enzymes
– Amylase and Diastase;
Immobilization and their applications in drug manufacture.
Biomass – Lactobacillus sporogenes
Immunization Products
Manufacture,
Standardization, Storage, Labeling and Specific Applications of the following
vaccines: Bacterial vaccines, toxoids, viral vaccines, Rickettsial vaccines,
Rabies, MMR, BCG, DPT, Cholera, Hepatitis B and Polio
Standardization
and Storage of the following Passive immunization products – Anti toxins, Anti
venom, Immune sera and other products related to immunity and Immuno
Diagnostics;
Blood and Glandular Products
Collection,
processing and storage of whole human blood, Concentrated human R.B.C. dried
human plasma, Human plasma protein fraction, dried human serum, Human
fibrinogen, Human thrombin, human normal immunoglobulin, Human fibrin foam,
Plasma substitutes – Ideal requirements, PVP, Dextran 40, Control of blood
products, Transfusion products.
Preparation
of extracts and isolation of pure substances and their dosage forms from
Pituitary, Adrenal, Pancreas and Thyroid glands;.
Biotransformations and Animal Cell Biotechnology
Microbial
transformation of steroids: Introduction, Types and methods of transformations
mediated by microorganisms, design of biotransformation processes and selection
of organisms.
Animal
cell culture: Techniques, Media used and Applications.
Hybridoma
culture: Production of monoclonal antibodies and their applications.