Biochemical
organization of the cell and transport processes across cell membrane.
The
concept of free energy, determination of free energy change from equilibrium
constant and reduction potential, energy rich compounds, production of A TP and
its biological significance.
Enzymes
- Nomenclature & classification, Kinetics, mechanism of action and
inhibition, clinical applications of enzymes, isozymes and coenzymes.
Carbohydrate
metabolism: - Glycolysis, gluconeogenesis, glycogenolysis, glycogen synthesis,
metabolism of galactose, role of sugar nucleotides in biosynthesis; pentose
phosphate pathway. TCA cycle, its significance, Anapleuritic reations, Effects
of inhibitor and regulation of TCA cycle, Glyoxalate cycle.
Lipid
metabolism - fate of dietary lipids; beta oxidation, oxidation of unsaturated
fatty acids; synthesis of ketone bodies, biosynthesis, of saturated and
unsaturated fatty acids, cholesterol metabolism, phospholipids and
sphingolipids.
Electron
transport and biologial oxidation. Nitrogen balance, metabolism of amino acids;
biosynthesis of purins, pyrimidines and their nucleotides, formation of uric
acid.
Integration
of carbohydrate, lipid and protein metabolism. Biosynthesis of RNA and DNA,
Physical and chemical mutagenesis, DNA repair mechanism, recombinant DNA,
mechanism of protein synthesis and its regulation, inborn errors in
metabolism.