Introduction
to the Science of Microbiology and Microscopy. Groups of microbes (bacteria, fungi,
virus and actinomycetes) classification, macro and micro morphology and
taxonomy. Different methods of bacterial count. Nutrition, Cultivation,
Isolation, Identification and Preservation of pure cultures. Organisms
important in Pharmacy.
Different
biochemical reactions employed in identification of organisms, stains and
staining, tolerance, Physiology and reproduction of bacteria, actinomycetes,
fungi, yeasts and viruses. Microbial genetics and Variation: Introduction,
genetic organization, mutation, mutagens, different types of mutants, physical
and chemical mutagenesis repair mechanism and their isolation.
Disinfections:
Factors influencing disinfections, dynamics of disinfections, different groups
of disinfectants and antiseptics and their evaluation and applications.
Sterilization:
Premises and Equipment, detailed evaluation and application of different
sterilization methods. Sterilization indicators and their importance.
Microbial
attack and host defense, virulence and pathogen city, primary and specific
defensive mechanisms of body.
General
Principles of immunology and their applications. Immunogenetics: Classification
and principles of different types of immunity, Immune systems - humoral
immunity, cellular immunity and tolerance. Phagocytosis, Hypersensitivity and
other reactions.
General
Principles of Serology and Chemical nature of antigens, antibodies. Different
antigen - antibody reactions and their applications. Precipitation,
agglutination and their significance in diagnosis and diagnostic tests.
Different antigens of bacterial cells, monophasic and biphasic variation.
Bacterial exotoxins and endotoxins, Toxoids.
General
principles of infection and communicable diseases. Significant symptoms,
General modes of transmission of the following epidemic and endemic
diseases.
a)
Tuberculosis, cholera, typhoid. b) Diphtheria, whooping cough. c) Plague,
malaria, filariasis, infuenza. d) Infective hepatitis, poliomyelitis.
Systematic studies of a few selected organisms -
E.Coli, Pencillium sps, Streptomyces sps, Saccharomyces sps. Microbiology of
water and milk.